WebApr 13, 2024 · We further demonstrate that EGFL7 knock-out in a Nestin-CreERT2-based mouse model produces a pronounced upregulation of neurogenesis within the subgranular zone. RNA sequencing identified that the increased expression of the cytokine VEGF-D correlates significantly with the ablation of EGFL7. WebAug 18, 2015 · The utility of the nestin-CreERT2/R26R-YFP mouse for inducible gene ablation in stem cells and their progeny in vivo in the two major regions of adult neurogenesis is highlighted. Expand. 465. PDF. View 14 excerpts, references methods and background; Save. Alert.
Stress-Induced Anxiety- and Depressive-Like Phenotype ... - PubMed
WebIn addition, various tissue specific CreERT2 mouse lines are widely available, making cell lineage tracing studies more specific and powerful. In this protocol, we provide a practical guide for researchers to map progeny of specific cells such as chondrocytes during development using a fluorescent reporter (tomato, red) and multiple chondrocyte Cre lines. WebJan 20, 2024 · Nestin-CreERT2 Line5-1 ; B6-Nes-CreER#5-1: H-2 Haplotype: ES Cell line: Background strain: C57BL/6JJcl: Appearance: Strain development: Developed by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2005. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. jrタワーホテル日航札幌 公式
Recombination in nestin-CreER T2 /R26R-YFP mice is
Weba constitutively expressed CreERT2 in the Rosa26 locus (Sox2fl/fl;R26 CreERT2/+ mice; Seibler et al., 2003, Taranova et al., 2006). We injectedmice with tamoxifen at9 months of age, confirmed that this caused recombination of the Figure 3. Lineage Tracing Demonstrates that Most NCPCs in the Regenerating Dermis Are Induced to Express … WebWe used Nestin CreERT2 mice to trace SC-NSCs descendants in the spinal cord of mice subjected to SCI. While mice carrying the suicide gene thymidine kinase (TK) along with the GFP reporter, under the control of the Nestin promoter regions (NestinTK mice) were used to label and selectively kill SC-NSCs. WebJan 22, 2015 · We used nestin-CreER T2 /tdTomato-reporter mice to trace the lineage of putative nestin-expressing cells and their progeny in the cochleae of adult mice. Nestin, … adi to vhd